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Studies On Sprouting, Germination And Seedlings Of Borassus Aethiopum, A Nigerian Palm.

IJATO J.Y

Department of Plant Science, Faculty of Science, University of Ado- Ekiti, Nigeria.

jamesyeni@yahoo.com

GSM: 08067335124

ABSTRACT: Studies were carried out on the sprouting, germination and seedlings of Borassus aethiopum to determine the most suitable medium in which the seeds / fruits of this palm can be sprouted . The seeds of Borassus aethiopum germinated by sending out cotyledon sheaths into the soil up to a length of 70cm without the emergence of the plumule. Ripe fruits of Borassus aethiopum used for the studies were collected from the vegetation reserve of University of Ado-Ekiti, Ado-Ekiti, placed in a polyethene bag and taken to the laboratory of the Department of Plant Science, University of Ado-Ekiti for further studies. An experimental site of the Department of Plant Science was used for the study, the site was harrowed and heaped to a height of 2 ½ feet, ten fruits each of Borassus   aethiopum were planted in each per treatment group. Some fruits were soaked in water for 15, 20 and 25 days dehusked while some remained undehusked, some were planted in the soil directly, some were just observed without planting in the soil, some were placed inside thick and transparent polyethene bags and observed for germination. Watering was done regularly and manual weeding was done to prevent weed invasion. Dehusked seeds of Borassus aethiopum soaked for 15 days required 16-20 days and 21-25 days to attain 30 and 40 percent cumulative germination respectively while soaking of dehusked seeds of Borassus  aethiopum for 20 and 25 days required the same number of days (6-10) to attain 20 percent cumulative germination, undehusked fruits of Borassus aethiopum irrespective of number of days of soaking did not support any germination. Dehusked fruits of B. aethiopum planted in the soil directly germinated faster at 50 percent within 11-15 days while the undehusked fruits required 16-20 days and 21-25 days to attain 40 and 50 percent germination respectively. Sprouting Borassus aethiopum fruits in polyethene bags was not successful irrespective of number of days under observation whether dehusked or undehusked.

[IJATO J.Y. Studies On Sprouting, Germination And Seedlings Of Borassus Aethiopum, A Nigerian Palm. Stem Cell. 2010;1(3):1-5] (ISSN 1545-4570). http://www.sciencepub.net. 1

doi:10.7537/marsscj010310.01

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Study of Some Lymphoproliferative Clonal Markers Following Renal Transplantation

Elham Ragab Abdel-Samea , Ahmad Shawki Mohammed, Farha Abdel-Aziz El-Chennawi

Clinical Pathology Department, Mansoura University, Egypt

elhamelngar@yahoo.com

Abstract: Post-renal transplant lymphoproliferative disorders (PTLDs) is a well known category among all the other lymphoproliferative disorders. The cause of this complication is mainly due to long term immunosuppression, and infection particularly by Epstein-Barr virus. The aim of this work is the early recognition of clonal changes for early therapeutic intervention as most of these changes are polyclonal. Yet the transition to oligo – and subsequent monoclonal is important for follow up with the other investigation to reach the proper diagnosis. This study compromised sixty post – renal transplantation patients , they were selected from impatient and outpatient clinics of Urology and Nephrology Center, Mansoura Faculty of Medicine. Those patients were compared to ( 10 ) de novo Non – Hodgkin lymphoma patients served as a positive control group. These patients were subjected to the following clinical laboratory studies; full history taking, clinical examination, Routine  laboratory investigation and Flow cytometry for CD19, CD20  and light chain immunoglobulin Kappa/Lambda. There is a high statistically significant increase in CD19 ( 41.2817 + 15.1199 ,21.5000 + 2.9533 ), CD20 ( 37.9917 + 14.7690 , 21.700 + 2.7909 ) in patient compared to healthy group respectively, there is a statistically significant difference in  Kappa/Lambda ratio ( 1.3240 + 0.6554 , 2.1210 + 0.4968, 1.6310 +  1.5354 ) in patients when compared to both the healthy group and the lymphoma patients. There is statistically significant decrease in CD19, CD20 in patients group in comparison to lymphoma patients ( P0.0001 ), also there is high statistically significant decrease in CD19, CD20 in healthy controls in comparison to lymphoma patients ( P0.0001 ). From these results, we conclude that some of these post – renal transplant patients had abnormal hematological findings in the form of oligoclonality , which may lead to subsequent hematological malignancies. These patients should undergo periodical flow cytometric analysis to reveal if there are any clonal changes.

Elham Ragab Abdel-Samea , Ahmad Shawki Mohammed, Farha Abdel-Aziz El-Chennawi. Study of Some Lymphoproliferative Clonal Markers Following Renal Transplantation. Stem Cell. 2010;1(3):6-] (ISSN 1545-4570). http://www.sciencepub.net. 2

doi:10.7537/marsscj010310.02

Keywords: Renal transplantation, lymphoproliferative disorders, Epstein-Barr virus, immunosuppression

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In Vitro Differentiation Of Bone Marrow – Derived Stem Cells Into Insulin Producing Cells

Noha El Sayed Attia ^(1),Prof. Dr. Mostafa M. Abou El Ela ^(1),Prof. Dr. Shefaa M. F. El Sawy ^(1),Dr. Ghada M Mourad Fouad ^(1),Dr. Mohammed I S Ahmed ⁽²⁾

(1) Department of Histology& Cell Biology, Faculty of medicine, Alexandria University.
(2) Department of Clinical Pathology, Faculty of medicine, Alexandria University.

ABSTRACT: Diabetes mellitus is a worldwide health problem. It constitutes a metabolic disorder that results, among many other causes, from dysfunctional pancreatic islet beta cells with inadequate release of insulin hormone responsible for the control of normal blood glucose level. As stem cells are the key to tissue regeneration and repair attention was directed towards the role of stem cells in regeneration therapy for diabetes.

Noha El Sayed Attia, Mostafa M. Abou El Ela, Shefaa M. F. El Sawy, Ghada M Mourad Fouad, Mohammed I S Ahmed. In Vitro Differentiation Of Bone Marrow – Derived Stem Cells Into Insulin Producing Cells. Stem Cell. 2010;1(3):15-16] (ISSN 1545-4570). http://www.sciencepub.net. 3

doi:10.7537/marsscj010310.03

Keywords: Diabetes mellitus; metabolic disorder; pancreatic islet; beta cell; insulin; hormone; blood; glucose; tissue; regeneration; therapy

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Numerical Analysis of Arched Strip Footings as A Foundation system of Bearing Walls Structures

Prof. S. S. Abdel-Salam¹, Ass. Prof. E.A. El- Shamy¹ and Dr. H.E. Abd-El- Mottaleb¹

 1 Dept. of Structural Eng., Faculty of Eng., Zagazig University, Egypt.

Corresponding author: E.A. El- Shamy E-mail address: Emanelshamy@hotmail.com

ABSTRACT: The construction of bearing walls structures does not necessarily require using reinforced concrete plane strip footings, but these structures can be constructed using arched plain and reinforced concrete strip footings. This paper aims at analyzing plain and reinforced concrete arched strip footings, as foundation system of bearing walls structures, as an alternative solution to reduce the construction cost of buildings. The effect of soil type, arched strip footing's height and the bearing walls vertical load on the dimensions and capacity of arched strip footings were studied in this paper. A numerical model for the non-linear analysis of arched strip footing-soil interaction problem based on the finite and infinite element was implemented. A computer program was developed to model the arched strip footing-soil installation. The material and geometrical non-linearity of the concrete strip footing taking into account the non-linear stress-strain relation of concrete and presence of cracking were also considered. In addition, Duncan-Mohr-Coulomb Modified model was used to simulate soil non-linearity. The obtained numerical results were compared with the traditional method in designing of strip footings commonly used by structural engineers. Design charts were proposed and presented for structural designers in order to calculate arched P.C & R.C strip footing dimensions according to soil type and vertical load for such strip footings which considerably cost less than traditional bearing walls construction system.

[S. S. Abdel-Salam, Ass. E.A. El- Shamy and H.E. Abd-El- Mottaleb. Numerical Analysis of Arched Strip Footings as A Foundation system of Bearing Walls Structures. Stem Cell. 2010;1(3):17-26] (ISSN 1545-4570). http://www.sciencepub.net. 4

doi:10.7537/marsscj010310.04

Keywords: Structural design, Plain and reinforced concrete, Arched Strip footing, Non linear - Finite element

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Antioxidants And Hematological Profile In Pregnancy

*¹ Iribhogbe O. I, ¹ Aigbiremolen A, ²Akpamu U, ¹Emordi J. E, ²Inegbenebor U, ²Nwoke E. O.

 ¹Department of Pharmacology &Therapeutics and ²Department of Physiology, College of

Medicine Ambrose Alli University Ekpoma, Edo State Nigeria.

Correspondence to: Dr. O. I Iribhogbe.

Department of Pharmacology and Therapeutics, College of Medicine, Ambrose Alli University Ekpoma, PMB. 14, Edo State Nigeria.

Email: oignis@yahoo.com; Mobile Phone No: +2348065794437

ABSTRACT:  This study aims to investigate the effect of supplementation with vitamin A, C and E on  hematological profile in pregnancy using Wister Albino rats. 85 adult female rats were procured from the animal house of the College of Medicine, Ambrose Alli University, Ekpoma. They were divided into 5 groups; a control group A, vehicle group B, vitamin A treated group C, vitamin C treated group D and vitamin E treated group E all having 5 animals each respectively. After pregnancy was confirmed, the control group A received distilled water, the vehicle group B received Tween-80 while the test groups C, D and E were dosed oro-gastrically with vitamin A, vitamin C and vitamin E at five different doses for eleven days respectively. Blood samples were collected 24hrs after completion of treatment and analyzed for Hemoglobin concentration (Hb), complete white blood cell (WBC) and differential count. Results were analyzed using SPSS version 17. Student t test was performed and a p value < 0.05 was considered significant. Results revealed that at a very low dose, vitamin A, C and E produced no significant change in Hemoglobin concentration. However, a dose dependent change was observed in the hematological profile. WBC count was lower in the experimental group when compared with control.  Polymorph count was significantly reduced in the test groups (p < 0.05) while lymphocyte count remained unchanged when compared with control (p > 0.05). Vitamin A, C and E cause a dose dependent alteration in hematological profile in pregnancy.

[Iribhogbe O. I, Aigbiremolen A, Akpamu U, Emordi J. E, Inegbenebor U, Nwoke E. O. Antioxidants And Hematological Profile In Pregnancy. Stem Cell. 2010;1(3):27-33] (ISSN 1545-4570). http://www.sciencepub.net. 5

doi:10.7537/marsscj010310.05

Key Words; Pregnancy, Hematological profile, Vitamins, Supplementation

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Mouse Stem Cell Literatures

Mark H Smith

Queens, New York 11418, USA

mark20082009@gmail.com

Abstract: The definition of stem cell is “an unspecialized cell that gives rise to a specific specialized cell, such as a blood cell”. Stem Cell is the original of life. All cells come from stem cells. Serving as a repair system for the living body, the stem cells can divide without limit to replenish other cells as long as the living body is still alive. When a stem cell divides, each new cell has the potential to either remain a stem cell situation or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, a bone cell, a nerve cell, or a brain cell. Stem cell research is a typical and important topic of life science. This material collects some literatures on mouse stem cell.

[Smith MH. Mouse Stem Cell Literatures. Stem Cell 2010;1(3):34-112] (ISSN 1545-4570). http://www.sciencepub.net/stem. 6

doi:10.7537/marsscj010310.06

Key words: stem cell; life; gene; DNA; protein; mouse

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