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Study on Characterization of thiol protease

¹Dr. Krishan Pal and ²Ankita Devi

¹Associate Professor, Department of Botany, OPJS University, Churu, Rajasthan (India)

²Research Scholar, Department of Botanu, OPJS University, Churu, Rajasthan (India)

e-mail: ankita39999panwar@gmail.com

Abstract: The Sepharose 6B column was packed as detailed under ‘Materials and Methods’. After proper equilibration with buffer A (0.1M sodium acetate buffer pH 5.5 containing 0.2 M NaCl and 1 mM EDTA), the void volume (V₀) and the total volume (V_t) of the Sepharose 6B column (90 x 0.8 cm) were determined as 36.0 ml and 115.0 ml, respectively. Various molecular weight marker proteins [2.0 mg each of carbonic anhydrase (29 k Da), bovine serum albumin (66 k Da), alcohol dehydrogenase (150 k Da) and β-amylase (200 k Da)] were dissolved in 2.0 ml of eluent buffer A. This mixture was loaded on the column which was eluted at a speed of 15 ml/h and fractions of 3.0 ml each were collected. The elution volume for each marker protein was calculated by estimating protein through absorbance at 280 nm. The purified thiol protease sample was eluted separately from the same column under identical conditions and the elution volume of the enzyme protein was calculated. The molecular weight of thiol protease was calculated from the standard graph plotted between log molecular weight against Ve/Vo which was found to be ~157 kDa.

[Krishan Pal and Ankita Devi. Study on Characterization of thiol protease. Rep Opinion 2019;11(11):1-3]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 1. doi:10.7537/marsroj111119.01.

Keywords: Thiol protease, Molecular weight, Sepharose, Gel filtration

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I understand and use of taiji map contribution sun chunwu

Sun Chunwu

Xiuxiu Garden, Xihu Town, Yangzhou City, Jiangsu Province, China 5008 Mainly engaged in the research of natural sciences, etc.; Name of the unit, Yangzhou Huading Electric, Jiangsu Province

Set Email: yzscw0514@163.com

Abstract: Knowing the material basis of the original celestial body, it is the force of the force to push forward the frictional motion. Its principle mechanism is to transform the sputum and copy more energy or force. The celestial body is from simple to complex. From the low-level to the advanced step-by-step evolution, the universe of all things or celestial bodies who are of great quality or proper method, who is a unified field in this living field. There is no immutability. They make a one-dimensional repetitive and orderly movement in time, and space is the orderly movement of disorderly movement, irregular velocity, irregularity, and asymmetrical elliptical motion. Explain that the universe's celestial bodies should start with the perfection of physical theory to solve the problem. Solved the physics theory, all the breakthrough problems have been solved. It is known that if a star moves upwards as one of the stars in the center of the galaxy, it will be consumed by the friction force on the field, and the inner core of the planet will be copied. The rotation force is reduced, and the rotation speed is slowed down. Therefore, a planet moving upwards forms a density wave when it is elliptical. The light transformed by the friction on the planet is also darkened, and it is shot at the center of the galaxy. And another ice age in winter.

[Sun Chunwu. I understand and use of taiji map contribution sun chunwu. Rep Opinion 2019;11(11):4-23]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 2. doi:10.7537/marsroj111119.02.

Key words: celestial body; matter; energy; force; eccentric vortex engine; planet; galaxies; center; orientation; heart vortex; elliptical motion

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Face Detection using open-CV and Python

Saima Kanwal, Abdul Sattar, Muhammad Farhan Arif, Jalil Abbas

Department of Computer Science , Lanzou University of Technology, China

Department of Computer Science & IT, Govt. College University Faisalabad (Layyah)

                                 School of Computer Science and Technology, Anhui University, China

Corresponding Author: sjshah786@gmail.com

Abstract--- The interest in computer vision has been popularised since a few decades. With the passage of time, its efficiency and accuracy of face detection is being improved day after day. As per the speed of computer is being rapidly increased but we are still willing for much better, similarly the speed of detecting a face is also being improved for Excellency. This document describes the concepts and techniques to get a face detected through a camera by using open-CV and Python. In this process, a neural network is used to get accurate results in face detection and there are different algorithms adopted for a neural network such as Deep Neural Networks (DNN), Convolutional Neural Networks (CNN), etc. Tensor-Flow recently uplifted by Google while working on Neural Networks, is the great tool to train data set in a better way having three layers named input layer, hidden layer and output layer. With the help of open source libraries an image or a frame can be detected if there present the specific functionalities the library is about, similarly face detection library also focusses the face in the image and to get more accuracy hence we have the concept of Artificial Neural Network (ANN).

[Saima Kanwal, Abdul Sattar, Muhammad Farhan Arif, Jalil Abbas. Face Detection using open-CV and Python. Rep Opinion 2019;11(11):24-28]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 3. doi:10.7537/marsroj111119.03.

Keywords: ANN, CNN, DNN, open-CV, Face, Detection, Python, Deep Learning

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A perspective review on green synthesis and characterization of silver nanoparticles (AgNPs) by Ocimum sanctum (tulsi)

Shahzad Sharif Mughal¹, Syeda Mona Hassan^1*, Nagina Shabbir¹, Maryam Mushtaq¹, Sumaira Perveiz¹, Muneeza Muneer¹, Faheem Abbas²

¹Department of chemistry, Lahore Garrison University, Lahore, Pakistan

²Department of chemistry, university of Agriculture Faisalabad, Pakistan

^*Corresponding author’s email: s.monahassan@lgu.edu.pk

Abstract: In green nanotechnology, an interest is developed among researcher to synthesis nanomaterial. Due to physico-chemical and biological properties of Ag nanoparticles synthesized by bio materials have diverse applications in various fields including biosensors, optoelectronics, drug delivery, and magnetic devices etc. This review emphasis the synthesis of silver nanoparticles by using tulsi Ocimum Sanctum leaves under the discipline of green chemistry. Green synthesis method is an eco-friendly method, cost efficient provides a potential to synthesis metallic nanoparticles by different plants. The use of plants for synthesis of Ag nanoparticles has fascinated the researcher to find out the metal uptake & mechanism involved in the reduction of Ag ions into Ag nanoparticles. All the nano particles have a size ranges from 1nm-100nm. To investigate the effect of tulsi and its derivative quercetin regards to the formation of Ag nanoparticles, some physico-chemical conditions are employed e.g., temperature, reactant concentration, pH, and reaction time. The main characterization of Ag nanoparticles generally investigated by various techniques includes UV-Visible spectroscopy, Mass spectrometry, X-RAY Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) etc. The characterizations reveal that synthesis of Ag nanoparticles by tulsi leaf and its derivative quercetin have the same optical, anti-bacterial and morphological characteristics. So the results demonstrate that quercetin present in tulsi is responsible for the reduction of Ag ion to Ag nanoparticles.

[Shahzad Sharif Mughal, Syeda Mona Hassan, Nagina Shabbir, Maryam Mushtaq, Sumaira Perveiz, Muneeza Muneer, Faheem Abbas. A perspective review on green synthesis and characterization of silver nanoparticles (AgNPs) by Ocimum sanctum (tulsi). Rep Opinion 2019;11(9):29-37]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 4. doi:10.7537/marsroj110919.04.

Key words: Green synthesis; Ag nanoparticles; Tulsi; quercetin; characterization.

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Assessment of knowledge, practice and factors associated with Brucellosis prevention among residence in Bati district, Oromiya Zone, Amhara state, Ethiopia

Hussein Mohammed (Msc in MPH), Dr. Tadesse Guadu (MPH), Mr Malede Fentie (MPH), Teleko Girma (MPH)

^1. university of Gondar College of medicine and Department of public health and veterinary medicine, Gondar, Ethiopia P.O. Box: 196

^2. university of Gondar College of medicine and Department of Environmental Health, Gondar, Ethiopia P.O. Box: 196

^3. university of Gondar College of medicine and Department of Public Health Biostatics, Gondar, Ethiopia P.O. Box: 196

⁴University of Gondar College of medicine and Department of Public Health and veterinary medicine, Gondar, Ethiopia P.O. Box: 196

 Email: husienmoha660@gmail.com

Abstract: This study was done on residents of Bati district farmers from April to May 2018 to assess the knowledge and practice to prevention of the disease in the area of study. Cross-sectional study design and multistage sampling procedures were employed to select households for this study. The data were collected from785 households, using pretested and structured questionnaire. Data entry was done by using Epi info version 7 statistical software and SPSS version20 soft ware were used for data analysis. Both Bivariable and multiple logistic regressions were fitted. P-value with 95％CI was used and p-value ＜ 0.05 were declared the significant association between knowledge and practice on brucellosis prevention and its associated factors. A total of 806 respondents were participated with a response rate of 97.4％ (785). Among those, 785 respondents 682(86.9％) of them were males. Moreover respondents those educated had 2.863 times more likely good knowledge of brucellosis prevention (AOR=2.863, 95%CI: 2.215-3.700) than those not educated. Respondents those that had shared homes with animals were 2.041 times less likely had good practice of brucellosis prevention (AOR=2.041, 95 %CI: 1.091-3.816).

[Hussein Mohammed (Msc in MPH), Dr. Tadesse Guadu (MPH), Mr Malede Fentie (MPH), Teleko Girma (MPH). Assessment of knowledge, practice and factors associated with Brucellosis prevention among residence in Bati district, Oromiya Zone, Amhara state, Ethiopia. Rep Opinion 2019;11(11):38-42]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 5. doi:10.7537/marsroj111119.05.

Keywords: Bati district, Ethiopia, Brucella, Knowledge, Practice and Associated factors

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Factors Related To Life Insurance Policy S Customers In District Karnal, Haryana (India)

Dr. Gulab Singh Parmar and Ms. Nutan

Assistant Professor, Department of Commerce, OPJS University, Churu, Rajasthan (India)

Research Scholar, Department of Commerce, OPJS University, Churu, Rajasthan (India)

e-mail: nutanahlawat.amity2009@gmail.com

Abstract: In the present study, the information of LIC in district Karnal. In the modern era business product should be advertised through many tools of modern marketing. Each and every institution advertise its product/service to the customers so that information can be obtained by the specified customer. As per table above that 24% customers got information from media about LIC in Karnal where as 17% of the customers got information of LIC in Karnal district by self effort. As per table 40% of the total survey customers got information in relation to types of insurance policy covered risk, benefits of insurance by agent, which are golden person in the insurance sector. Therefore, as we know insurance is backbone of each/every insurer as well as persons.

[Gulab Singh Parmar and Ms. Nutan. Factors Related To Life Insurance Policy S Customers In District Karnal, Haryana (India). Rep Opinion 2019;11(11):43-49]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 6. doi:10.7537/marsroj111119.06.

Keywords: Respondents, Behavioral Study. Life Insurance Company, Karnal, Haryana.

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Liner Shipping and Container Terminal Performance of Apapa and Onne Ports in Nigeria

Odiegwu, Chinwendu Laurentia

M. Sc. (Shipping Management) Scholar, Maritime Studies, Faculty of Management Sciences, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria

Email: codiegwu2009@yahoo.com

Abstract: This study investigated the relationship between liner shipping and container terminal performance of Apapa and Onne ports, Nigeria. Liner shipping served as the independent variable or predictor variable. Also, container terminal performance served as the key dependent variable or criterion variable under which the measures such as cargo throughputs and vessel turnaround time have been appraised. The population of the study consisted of the staff in the two ports (Apapa,636 and Onne, 277), that is 913 staff and the study sampled 279 respondents out of which 222 of them were found useful and valid for the study analysis. The study collected data with the help of a structured questionnaire. The study used Pearson Products Moment Correlation Coefficient (r) to test the hypotheses with the aid of SPSS 22.0. The reliability of the research instrument was tested using the Cronbach alpha to ascertain the reliability of the instrument. The study found that liner is very instrumental to effective shipping operations. Conclusively liner shipping has a positive and significant relationship with cargo throughputs of Apapa and Onne Ports and liner shipping has a positive and significant relationship with vessel turnaround time of Apapa and Onne Ports. Therefore, the study recommends that port operators’ performance should be appraised constantly in order to ensure that the maritime sector is positioned to achieve the stakeholders’ objectives in Nigerian ports.

[Odiegwu, Chinwendu Laurentia. Liner Shipping and Container Terminal Performance of Apapa and Onne Ports in Nigeria. Rep Opinion 2019;11(11):50-72]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 7. doi:10.7537/marsroj111119.07.

Keywords: Liner Shipping, Container Terminal Performance, Cargo Throughputs, Vessel Turnaround Time

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Review Of Literature On Study On Irrigation In Various Districts Of State Haryana (India)

¹Dr. Kalu Ram and ²Mrs. Baby Rani

¹Assistant Professor, Department of Geography, OPJS University, Churoo-331303, Rajasthan (India)

²Research Scholar, Department of Geography, OPJS University, Churoo-331303, Rajasthan (India)

²CC email: navjot@ewit.in

Abstract: One of the basic and important objectives of the canal irrigation system is the delivery and distribution of water for irrigation among the water users. Due to the shortage of water in the system, the water is delivered to different parts of the canal system in rotation, i.e., using the ‘Rotational Programme of Channels’ (Gustafson and Reidinger, 1971). Supplies of irrigation water to the farmers have been awarded in the past by the system of 'warabandi' based on equitable and proportionate water allowance. Warabandi is a rotational and proportional method for equitable allocation of the available water in an irrigation system. The twin objectives of warabandi have 8 been high efficiency and equity in water use and both objectives are to be achieved and guaranteed by self-policing rotation system (Bandaragoda, 1998). Further, warabandi is agreed upon by concerned farmers/users and appropriate canal authority. Though this system of distribution is intended to be fair but the unpredictability of water supply is being faced by the users. The problem of water supply unpredictability arises due to little or no institutional control mechanism over the rotation and rationing of water based on non-market mechanism. It is also clearly evident that trade-off between hydro power generation and irrigation, reservoir factors and capacity factors contribute to the uncertain water supply (Gustafson and Reidinger, 1971). Presently, in Punjab warabandi system of canal water distribution and allocation among the water users is in operation. Further, mainly three types of warabandi are being used, namely: khuli–wari (open turn), panchayati-wari, and weekly-wari. Field staff of the Irrigation wing fixes the turn, duration and quantity of water for farmers. The basis for the distribution and allocation of water depends upon the size of the land holding, distance from the outlet (mogga), and certain other factors. That is the canal water distribution and allocation in Punjab has been carried out with little modifications in the warabandi system. Now attempt is being made to allocate and distribute water more scientifically through computerization of the distribution and allocation process.

[Ram, K. and Rani, B. Review Of Literature On Study On Irrigation In Various Districts Of State Haryana (India). Rep Opinion 2019;11(11):73-84]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 8. doi:10.7537/marsroj111119.08.

Keywords: Literature, Intensity, Crop Rotation, Irrigation, Haryana, India

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Assessment of Knowledge, practice and its associated factors towards Rabies prevention among residents in North Wollo Zone, Ethiopia

Telek Girma (Msc in MPH) ₁, Dr. R P Raju (Associate professor) ², Mrs. Melkitu Fentie (MSc in MPH) ³

^1.university of Gondar College of medicine and Department of public health and veterinary medicine, Gondar, Ethiopia, P.O. Box: 196

^2.university of Gondar College of medicine and Department of Environmental Health, Gondar, Ethiopia, P.O. Box: 196

^3.university of Gondar College of medicine and Department of Public Health Nutrition, Gondar, Ethiopia, P.O. Box: 196

Email: telekgirma12@gmail.com

Abstract: This study was done in North wollo Zone, Ethiopia, from March 2018 to June 2018 to assess the knowledge and their practice to prevention of the disease in the area of study. Cross-sectional study design and multistage sampling procedures were employed to select households for this study. The data were collected from 785 households using pretested and structured questionnaires. Interviewer administered structured and pre-tested questionnaire was used to collect socio-demographic, knowledge and practice for prevention of rabies and related variables. Data entry was done using Epi info version 7 statistical software. SPSS version 20 soft ware were used for data analysis. Both binary and multiple logistic regressions were fitted. P-value with 95％CI was used and p-value < 0.05 were declared the significant association between knowledge and practice on rabies prevention and its associated factors. A total of 806 respondents were selected with 97.4％ (785) response rate. Among those, 785 respondents 671(85.5％) of them were males and 114(14.5％) were female. Respondent that had good awareness about rabies had more likely good knowledge than that of respondent that had low rabies awareness (AOR=4.45, 95% CI: 1.068-18.580). Respondents that did not use post exposure prophylaxis were 0.002 times unlikely less than that of respondents with good practice that used PEP. (AOR=0.002,0.001-0.019:, 0.001).

[Telek Girma (Msc), Dr. R P Raju (Associate professor), Mrs. Melkitu Fentie (MSc). Assessment of Knowledge, practice and its associated factors towards Rabies prevention among residents in North Wollo Zone, Ethiopia. Rep Opinion 2019;11(11):85-90]. ISSN 1553-9873 (print); ISSN 2375-7205 (online). http://www.sciencepub.net/report. 9. doi:10.7537/marsroj111119.09.

Keywords: Bugna, Gidan, Knowledge, Lalibela, Lasta, Practice, Rabies

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