Report and Opinion
Volume
2 - Issue 8, Cumulated 14, August 25, 2010, ISSN 1553-9873
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CONTENTS
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Titles / Authors
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1
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Isolation of lactic acid bacteria from kantong, a condiment
produced from the fermentation of kapok (Ceiba pentandra)
seeds and cassava (Manihot esculentum) flour
Elmer Nayra Kpikpi a, b,
Richard L. K. Glover a, Victoria Pearl Dzogbefia
b, Dennis Sandris Nielsen cMogens Jakobsen
c.
a
Department of Applied Biology,
Faculty of Applied Sciences, UDS P.O. Box 1350 Tamale N/R, Ghana
b
Department of Biochemistry and Biotechnology Faculty of
Biosciences, Kwame Nkrumah University of Science and Technology,
Kumasi, Ghana.
c
Department of Food Science, Food Microbiology, Centre for
Advanced Food Studies (LMC), Faculty of Life Sciences,
University of Copenhagen, Rolighedsvej 30 1958, Frederiksberg C,
Denmark
elmer_kpikpi@yahoo.com
Abstract:
Kantong, a traditional food
condiment of the people of Northern Ghana, is produced by
fermentation of Ceiba pentandra seeds and cassava flour. Knowledge of the microbiology of the fermentation process will
be useful in its technological improvement and starter culture
development. There was a drop in the initial pH from 6.9 before
fermentation to 4.9 after fermentation with change in color of
the product from grayish to dark brown as well as the
development of a more desirable flavor.
Lactic acid bacteria (LAB) with counts between 106
and 109 cfu/g were isolated on MRS agar and subjected
to Gram, catalase and oxidase tests. The LAB were further
identified by biochemical and genotypic methods using rep-PCR,
(GTG)5 primer, 16S rRNA gene sequencing and
carbohydrate assimilation profiling. A total of 331 Lactic acid
bacteria were isolated of which 47% were Lactobacillus
plantarum, 18% Lactobacillus fermentum, 8%
Leuconostoc mesenteroides, 12% Pediococcus acidilactici
and 15% as Lactobacillus brevis. [Report and Opinion
2010;2(8):1-7]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.01
Key words: fermentation; condiment; lactic acid bacteria;
Ceiba pentandra |
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Retanning Agents for Chrome Tanned Leather
based on Nano-Emulsion of Styrene/Butyl Acrylate Copolymers
EL-Shahat H.A. Nashy (a)*,
Ahmed I. Hussein (b) and Mohamed M. Essa (b)
Chemistry of Tanning Materials
and Leather Technolog(a),
Polymers and Pigments(b) Departments,
National Research Center, Dokki, Giza 12622, Egypt
E mail:
Ismaelahmed1@yahoo.com
Abstract: Retanning
process is one step of wet-finishing operations and is very
important operation which overcomes the disadvantages of chrome
tan. For this purpose two different nano-emulsions of
styrene/acrylate copolymers have been prepared to be used as
retanning agents. The main difference and characters of the two
nano - copolymers were studied. The particle size of the two
prepared co-polymers was proved by transmission electron
microscope (TEM). The influence of the two prepared copolymers
on chrome tanned leather as retanning agents was also studied.
The properties of the retanned leather, namely, tensile strength
and elongation at break were measured. Thermal stability and
texture of grain surface and fibers were examined using thermal
gravimetric analysis (TGA) and scanning electron microscope (SEM),
respectively. All these parameters were the main target of this
work and used to evaluate the applicability of copolymers as
efficient ratannang agents. The retanned leather was achieved
with an improvement of mechanical properties, enhancement of
thermal stability, uniform dyestuff, softness and firmness
grain. [Report and Opinion 2010;2(8):8-15]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.02
Key words:
Retanning agent, Chrome tanned leather, Nano-particles,
Copolymers, Emulsion, Scanning electron microscope, Thermal
stability, transmission electron microscope, Mechanical
properties, Color difference |
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Performance Characteristics and Haematology of
Laying Birds fed Safzyme® Supplemented Soybean Hull
Diet
Babington Onyemachi Esonu1,
Oscar, Okechukwu Michael Iheshiulor2,
Okwunna, K. Chukwuka1, Apeh Akwu Omede1*,
Ifeanyi Princewill Ogbuewu1
1Department
of Animal Science & Technology, Federal University of
Technology, Owerri, P.M.B. 1526, Imo State, Nigeria.
2Department
of Animal Science, P.O.Box 338, 6700 AH
Wageningen, Netherlands.
*corresponding author:
jabulaniapeh@yahoo.com
Abstract:
A twelve- week feeding trial was
conducted to evaluate the effect of Safzyme® supplementation on
performance characteristics and haematology of laying birds fed
soybean hull diets. Three experimental layer diets were
formulated incorporating soybean hull meal at 0% (without
Safzyme® supplementation), 30% (without Safzyme®
supplementation) and 30% (with Safzyme® supplementation). Thirty
six Harco layers, 5 months into lay were divided into three
treatment groups and randomly assigned to three treatment diets
in a completely randomized design. Data were collected on feed
intake, body weight gain, hen-day production, egg size and
weight, internal indices of eggs, internal organ characteristics
and haematological indices. Results from performance studies
revealed that supplementing soybean hull diet with/without
Safzyme® significantly (P<0.05) affected performance, egg
quality indices, carcass characteristics and haematology
compared to the control. Also, hens fed soybean hull diet with/
without Safzyme® supplementation had increased weight of gizzard
and increased feed intake (P<0.05). The results of this trial
suggest that 30% dietary level of soybean hull meal with/without
Safzyme® supplementation could be used in laying birds diets
without any deleterious effects on birds. [Report and Opinion
2010;2(8):16-21]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.03
Keywords:
soybean hull meal, safzyme®, nutritive value, laying birds |
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Performance of Finisher Broilers fed varying
levels of raw (sun-dried) Cocoyam (Xanthosoma sagittifolium)
Corm Meals
Uchegbu, M. C., Omede, A.A.,
Chiedozie, J. C., Nwaodu, C. H. and Ezeokeke, C.T.
Department of Animal Science and
Technology, Federal University of Technology, PMB 1256, Owerri, Imo State,
Nigeria.
*corresponding author-
jabulaniapeh@yahoo.com
Abstract:
A 35-day study was
carried out using one hundred and twenty (120) four-week-old
marshal broiler strain birds to determine their performance when
fed varying levels of raw sundried cocoyam (Xanthosoma
sagittifolium) corm meals. Four broiler finisher diets were
compounded to contain 0%, 5%, 10% and 15% levels of the cocoyam
meal for T1, T3, T3 and T4
respectively. The birds were randomly divided into four groups
of thirty (30) birds each and each group subdivided into three
replicates of 10 birds per replicate in a completely randomized
design. The result showed that the birds on 0%, 5% and 15% based
diets had similar (p>0.05) body weight gains or 10% cocoyam corm
meal. There is a general increasing trend in the average daily
feed intake as the level of cocoyam meal inclusion increased up
to 15%. The birds on 15% cocoyam corm meal had a significantly
(p<0.05) higher feed intake (130.17 g/bird) than the feed intake
(120.39 g/bird) of the control. That is to say, at 15% cocoyam
corm meal level, the antinutritional contents were not high
enough as to depress feed intake in finisher broilers. The
various diets T1, to T4 were well utilized
by the various groups of birds going by the general low feed
conversion ratio values which ranged from 2.20 to 2.51. However,
the lowest cost of feed per kg broiler was achieved with T3
(10% cocoyam meal), producing at N93.87 per kg weight
gain. [Report and Opinion 2010;2(8):22-25].
(ISSN: 1553-9873).
doi:10.7537/marsroj020810.04
Key words:
Cocoyam (Xanthosoma sagittifolium), corm meal, Finisher
broilers, Performance |
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Agriculture Share of the Gross Domestic
Product and its Implications for Rural Development
Anyanwu Sixtus O1*,
Ibekwe U.C.2, Adesope Olufemi M3.
1. Department of Agricultural
Economics, Rivers State University of Education, Port Harcourt,
Nigeria
2. Department of Agricultural
Economics, Federal University of Technology Owerri, Nigeria
3. Department of Agricultural
Economics and Extension, University of Port Harcourt, Nigeria
sixtusanyanwu@yahoo.com
Abstract:
The study considered the
relationship between Gross Domestic Product and output of major
stapled food crops of Nigeria between 1990 and 2001. Data used
were collected from various issues of Central Bank of Nigeria
Statistical Bulletin, Annual Reports and Bureau of Statistics.
Using correlation matrix, the result showed that there is a
strong, positive and statistically significant relationship
between GDP and these food crops except for wheat. Policies
should therefore be put in place by the appropriate authorities
geared towards providing production incentives to the rural
farmers to enable them produce more of these stapled food crops
that have significant impact on the GDP of Nigeria. [Report and
Opinion 2010;2(8):26-31]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.05
Key words: Gross Domestic Product; Rural Development |
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Determinants of income among farm households
in Orlu Agricultural Zone of Imo State, Nigeria
IBEKWE, U.C
Department of Agricultural
Economics
Federal University of Technology,
pmb 1526, Owerri,
Imo State, Nigeria
omadesope@yahoo.co.uk
Abstract:
Worsening income distribution with economic growth is evident
in Nigeria. To provide an environment that will stimulate
growth and also ensure an equitable income distribution needs
a careful study of income determinants that will be used in
carefully tailored policies. This study determined an average
farm household income of N60,197.81 per annum and a per capita
income of N7,524.73. also, a Gini-coefficient of 0.488 was
determined. The income regression parameter estimates showed
that the variables, extension services property income and farm
size were positively correlated with farm household income and
were also significant at five percent. The variables income
from pension, hours spent on farm income from handicraft
education of household head, income transfers and age of
household head were positively correlated with farm household
income but not statistically significant at five percent. The
hypothesis of no significant difference in the contributions of
the determinants of farm household income was tested and
rejected at five percent level. It is suggested that a
careful integration of these income determinants in rural
development policies will no doubt improve the farm households
purchasing power as well as the income distribution in the
study area. [Report and Opinion 2010;2(8):32-35]. (ISSN:
1553-9873).
doi:10.7537/marsroj020810.06
Key words:
Income, Farm Household |
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Management of Job-Related Teacher Burnout in
Nigerian Schools
Lekia Nwikina1, Anthonia
Nwanekezi2
1. Department of Educational Management, University of Port Harcourt
2. Department of Curriculum Studies, University of Port Harcourt
omadesope@yahoo.co.uk
Abstract:
The paper helps teachers in Nigerian schools manage burnout, an
emotional exhaustion from excessive demands on their energy,
strength, and resources. It helps them, to understand the
burnout syndrome, the causes, symptoms, prevention and
remedies. It emphasizes that burnout is experienced by many
Nigerian school teachers and that it need not be a silent
disease. It recommends that further research is necessary to
document its incidence upon the teacher’s physical condition and
the student’s education. Exposing its causes, symptoms, and
types of prevention is vital if it is to be eliminated among
teachers. It recommends also that management should hold regular
meetings that can be used for staff support; should foster a
sense of teamwork among the staff; restructure jobs so that
teachers do not unduely spend as much time with particularly
demanding students and assignments. Workshops in stress
management or time management should be mounted regularly.
[Report and Opinion 2010;2(8):36-43]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.07
Key words: Job-Related Teacher Burnout, Nigerian Schools |
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Micropropagation Of Prosopis Cineraria
(L.) Druce – A Multipurpose Desert Tree
Surender Kumar And Narender Singh
Department Of Botany, Kurukshetra University
Kurukshetra (India)
E.mail-
suren_kr@yahoo.co.in
ABSTRACT:
A protocol has been developed
for micropropagation of a multipurpose desert tree species
Prosopis cineraria under in vitro conditions. Nodal
explants from mature plant of Prosopis cineraria were
taken and cultured after surface sterilization. One to multiple
shoots were induced on Murashige and Skoog,s(1962)
medium supplemented with various cytokinins and auxins
individually and in various combinations. 5.0 mg/l BAP and
Kinetin were found to be effective individually. 5.0mg/l BAP
+1.0mg/l IAA responded batter than all other media combinations.
Rooting of in vitro regenerated shoots(>2.5cm long) was
achieved on half strength MS medium supplemented with 3.0 and
5.0 mg/l IBA. In vitro regenerated plantlets were
transferred in pots containing sterilized sand and
vermiculites(1:1).After four weeks plantlets were acclimatized
to field conditions successfully with 60% survival rate. The
survived plants grew normally.
[Report and Opinion 2010;2(8):44-47]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.08
Key words-
Micropropagation,
Nodal explants, Prosopis cineraria, Axillary shoots |
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Farmers’ Sustained Adoption Decision Behaviors of Maize/Cassava
Intercrop Technology in Imo State: Lessons for Extension Policy
Development
F.N. Nnadi1and C.D. Nnadi2
1Dept.
of Agric. Extension, Federal University of Technology, Owerri
2Dept.
of Agric. Economics and Rural Sociology, Niger Delta University,
Bayelsa State.
Correspondence: dr_charleso@yahoo.com
Abstract: Various variables
influence farmers’ sustained adoption decision behaviors. Thus
the rate of sustained adoption of maize/cassava intercrop
technology, reasons for the sustenance and the determinants were
empirically investigated in Orlu zone of Imo State with a view
to drawing lessons for extension policy development. Data were
collected from 115 randomly selected crop farmers using
structured questionnaire. These were analyzed with percentage
counts, frequency tables and logit regression model at 0.05
level of significance. The farmers’ sustained the adoption of
the technology by 88.7% with reasons ranging from environmental
friendliness to full utilization/maximization of agricultural
land. The farmers’ age, family size, education, farming
experience, social organization membership, farm size, and
annual farm income as well as number of technological
information sources used determined their sustained adoption
decision behaviors. It was recommended that extension education
sensitization campaign be intensified using multi-media as well
as extension intervention strategies being guided by the socio –
economic attributes of the farmers. [Report and Opinion
2010;2(8):48-53]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.09
Keywords:
farmer; maize; cassava; intercrop technology |
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Application of some medicinalPlants to eliminate Trichodina
sp. in tilapia (Oreochromis niloticus)
Ahmed I.E.
Noor El Deen* and Razin, A.Mohamed.**
*Hydrobiology
Department, Veterinary Division, National Research Centre
**Medicinal and Aromatic Plants Department (MAP),National Research Centre
dr_ahmednoor2002@yahoo.com
Abstract:
Medicinal
plants are important elements of traditional medicine in
virtually all cultures and product promise a cheaper source for
therapeutics, greater accuracy than chemotherapeutic agents and
a viable solution for all problems which groupers culture faces
today.
The control of Trichodinaiasis and Aeromonus hydrophila in ponds
of stocked tilapia with any antiprotozoal and antibacterial
agent at present is evidently a cost. In addition, possibly
leaves toxic residues in tilapia and mortality. Also, affect for
a short times. For the previous reasons, the medicinal plants
instead. Garlic (Allium sativum) and Artemisia vulgaris as
optional medicinal plants to treat fish Trichodina sp and
Aeromonas hydrophila. 350 Tilapia O.niloticus (average
weight 100 ±20 g) derived from a private fish farm in Kafr El
Sheikh governorate infested with Trichodina sp, were kept in
cement ponds (3×8 metre) and
sex diets were
formulated to contain different levels of Allium sativum extract
(1, 4 and 8 g/kg diet) and Artemisia vulgaris extract (1, 3, and
4.5g/kg diet) added.
The results showed that crude extracts of either garlic or
Artemisia vulgaris at 800 mg/l significantly (P < 0.05)
eliminated Trichodina sp and Aeromonas hydrophila infections in
tilapia garlic and Artemisia vulgaris will be used as an
alternatives to chemicals to treat of infected
tilapia with Trichodina and Aeromonus sp.
[Report and Opinion 2010;2(8):54-59]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.10
Key
words:
Trichodina sp, Aeromonas hydrophila, tilapia, medicinal plant,
garlic, Artemisia vulgaris |
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A Study on Fish
pathogenic fungi and its periodicity in Tunga river of Karnataka
(South India)
Prasad Devi and Rajanaika
Department of P.G. Studies and
Research in Applied Botany, School of Biological Science,
Kuvempu University, Jnana Sahyadri, Shankaraghatta-577 451,
Shimoga Dist, Karnataka State, India
prasad.bhujanga@gmail.com,
rajump.nayak@gmail.com
Abstract:
A riverine ecosystem consists of a wide range of diversified
flora and fauna. The microflora in the riverine ecosystem is
dominated by Algae, Bacteria and fungi. Fungi in the riverine
ecosystem play an important role, they vitally link the food web
primarily as saprophytes and secondarily as pathogens. Tunga
river ecosystem consists a diversified flora of aquatic
phycomycetes, in which majority of them are Chytrids and
Oomycetes. These fungi are zoosporic in nature and some of them
mainly predate on Ichthyofauna and other riverine fauna. Aquatic
phycomycetes are the primitive fungi and they are at the bottom
of evolutionary series and they have their significance as
pathogens, combating them in the riverine ecosystem has become a
mammoth’s task. During monsoon and winter the fisher mans face
severe loss in fish productivity majority of them are due to
fungal infections on fishes and on their eggs. Tunga river
traverse a distance of 147 Km in Shimoga where the major fishing
points are located in the right bank of some important Taluks
viz., Thirthahalli, Mandagadde, Gajanur and Shimoga. In monsoon,
these fishing docks face a considerable decline in Fish catch.
Hence, sustainability and economical criteria of fish production
in Tunga river adverse the fisher mans lively hood. In this
scenario a detailed investigation was undertaken to identify the
occurrence, periodicity and disease incidence of the
phycomycetes fungi in the riverine ecosystem.
[Report and Opinion
2010;2(8):60-63]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.11
Key
words:
Aquatic phycomycetes, Fish pathogenic fungi, oomycetes, Fish
mycosis |
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EFFECT OF BURNING ON SOIL PHYSICAL PROPERTIES
IN THE DRY SUB-HUMID SAVANNA ZONE OF NIGERIA
S.
A. Pantami1, N. Voncir2 and
G. A. Babaji2
1Department
of Soil Science, Bayero University, Kano, Nigeria.
2Crop
Production Programme Abubakar Tafawa Balewa University, Bauchi,
Nigeria.
E-mail:
salihupantami@yahoo.com
Abstract:
A study was carried
out to assess the effects
of burning on the physical properties of soil on soils obtained
from within the dry sub-humid savanna agro-ecological zone of
northern Nigeria. Surface (0-15cm) composite soil samples were
collected from selected locations comprising Bauchi, Gombe and
Kano states from un-burnt, lightly burnt and intensely burnt
sites in farmers’ farms during land preparation of the 2007/2008
cropping season. The samples were obtained from both upland and
lowland areas and analysed in the laboratory to assess the
effect of burning on the soil’s physical properties. The
collected data were analysed using the SAS statistical software
to test for significant differences at 5% level of significance.
Where significant differences were found, the means were
separated using the Least Significant Difference (LSD). Results
of the laboratory analysis revealed that the soils within the
study area are sandy loam in texture, and burning has no
significant effect on the particle size distribution. Bulk
density and water holding capacity were seen to be significantly
affected as a result of burning. Bulk density increased from a
mean value of 1.35gcm-1 to 1.53gcm-1 as a
result of zero-burning and intense burning respectively within
the lowland areas and from 1.4135gcm-1 to 1.5635gcm-1
for the same treatments within the upland areas. The water
holding capacity was observed to reduce from a mean value of
37.47% to 35.90% within the lowland areas while within the
upland areas the reduction was from a mean value of 35.44% to
28.85% also for similar treatments. [Report and Opinion
2010;2(8):64-70]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.12
Keywords:
burning; soil; agro-ecological zone; Nigeria |
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Role of Fulvic Acid on Reduction of Cadmium
Toxicity on Nile Tilapia (Oreochromis niloticus)
Ahmed E. Noor EL Deen, Mona
S.Zaki and Hussan A. Osman
Hydrobiology Department,
Veterinary Division, National Research Centre, Dokki, Giza,
Egypt
dr_ahmednoor2002@yahoo.com
Abstract: Cadmium
is one of the most toxic heavy metal, enters the environment
from natural sources and as a result of mans activity. Tilapias
have the capability of concentrating metals by feeding and
metabolic processes, which can lead to accumulation of high
concentrations of metals in their tissues. The reduction of
toxic elements like cadmium in aquatic environments is needed by
any acceptable method. The effect of fulvic acid (FA) on cadmium
(Cd) toxicity and the impact on fish immunological,
hematological changes in Nile tilapia (Oreochromius niloticus)
were studied. The fish (100±10 g) were exposed to 10 ppm Cd
alone or with 0.1, 0.2 and 0.3 ppm for 15 and 45 days. Cd
exposure reduced significantly (P<0.04) such as erythrocyte
count (RBCs), haemoglobin content (Hb), haematocrit value (Hct),
mean cell haemoglobin (MCH) and mean cell haemoglobin
concentration. These parameters were improved when FA was
applied with Cd. The values of RBCs, Hb, Hct, MCH and MCHC were
increased significantly to be as in the control fish group. Addition of FA to Cd contaminated medium considerably reduced
metal absorption and accumulation in fish tissues, while it was
increased metals in water and feces. The present study, is
recommended that an optimum dosage of 0.3 g fulvic acid /l can
effectively chelate Cd from contaminated fish and water. [Report
and Opinion 2010;2(8):71-76]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.13
Keywords:
Nile tilapia, Cadmium, immunological, fulvic acid, hematology,
liver, gills, musculature |
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Green tea extract Role in removing the Trichodina
sp. on Oreochromis niloticus fry in the Egyptian fish
hatcheries
Ahmed Ismail Elsayed. Noor
El- Deen
Hydrobiology Department,
Veterinary Division, National Research Centre, Dokki, Giza,
Egypt
dr_ahmednoor2002@yahoo.com
Abstract:
Trichodina sp. are the most common ciliates parasite present on
the skin of pond-reared fish. The green tea extract (GTE) can be
economical valuable and effective to remove the ectoparasitic
ciliate Trichodina sp. from the skin and fins of infested
Oreochromis niloticus (O. niloticus ) fry . Bath of
infested O.niloticus fry (body weight 0.2–0.9 g) exposed
to 0.05% GTE for 15 minute ( min) or 0.9% GTE for 5 min resulted
in a decrease of 80 % and 95 % of the number of parasites on the
skin (dorsal and anal) and fins in comparison to 70 % untreated
controls (statistically significant, p < 0.01).No parasites were
present on the skin surface of infested O.niloticus 24
hours after baths . Neither the long (0.05 % GTE for 15 min) or
short bath treatment (0.9% GTE for 5 min). No mortality was
observed in the normal fry. Large-scale application of the
treatments with GTE (60,000 O.niloticus fry, 0.2–0.4 g)
infested with the parasite in hatchery showed significant
decreases of 80 % or 95 %, 0.05 % GTE for 15 min or 0.9 % GTE
for 5 min respectively. Cumulative mortalities for 48 h after
the treatments were 0.08–0.15 %. The 5 min bath with 0.9% GTE
was more efficient than the 15 min bath with 0.05% GTE, as
estimated from the results at ponds. This study concluded that
GTE treatment of infested fry of O. niloticus is
economic, safe, effective, and applicable to treatment of large
numbers of fish in hatchery. [Report and Opinion
2010;2(8):77-81]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.14
Key words:
Nile tilapia, Green tea extract ;hatchery ; Trichodina sp;
O.niloticus; fry |
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Plasmid Profile and Protease
Activity of β-Lactams Resistant Thermotolerant Soil
Isolate
B.
cereus BC2 from the Bacillus cereus Group
Species
Abdel-Shakour E.H.1*
and Roushdy M.M.2
1,2.
Botany and Microbiology Department, Faculty of Science, Al-Azhar
University, Cairo, Egypt
*essam_hussain@hotmail.com
Abstract:
The aim of the current study was
to isolate and investigate one of the most common genera of Gram
positive spore forming bacteria, genus Bacillus.
Bacillus cereus BC2 was grown well on nutrient agar
plates (pH 8) after 24 hrs of incubation at 55oC.
This isolate was
characterized by producing extracellular
protease which showed
activity at 55°C both
in vivo & in vitro.
The optimum enzyme production was at pH 8, incubation
temperature 55°C, and NaCl concentration of 5% (w/v) where the
highest protein concentration and the highest enzyme activity
were recorded. The activity of the partially purified enzyme
reached to 160.00 UmL-1
with about 3.10 mg mL-1 protein concentration
recorded. The isolate
Bacillus cereus BC2 was resistant to the β-Lactam
antibiotics tested including ampicillin. Finally, plasmid DNA
extraction from the isolate under study
and visualization through agarose
gel electrophoresis showed the absence of any free plasmid
molecules within the vegetative cells of this isolate.
[Abdel-Shakour E.H. and Roushdy
M.M. Plasmid Profile and Protease Activity of β-Lactams
Resistant Thermotolerant Soil Isolate B. cereus
BC2 from the Bacillus cereus Group Species.
Report and Opinion
2010;2(8):82-94]. (ISSN: 1553-9873).
doi:10.7537/marsroj020810.15
Keywords:
Plasmid; Protease; Antibiotic Resistant;
Thermotolerant; Bacillus cereus |
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