Welcome to send your manuscript to: sciencepub@gmail.com

No.

Titles / Authors

Full

Text

No.

1

Intracytoplasmic sperm injection outcomes and gene profile alteration in human cumulus cells related to advanced maternal age

Lili Wei ^1*, Jiaming Zhang ^2*, Liuhong Yang ¹, Wenting Zhou ³, Nan Li ⁴

^1. Department of Reproductive Medicine Center, Guangzhou Women and Children’s Medical Center Liuzhou Hospital, Liuzhou, Guangxi Autonomous Region 545006, People’s Republic of China

^2. Department of Laboratory Medicine, Guangzhou Women and Children’s Medical Center Liuzhou Hospital, Liuzhou, Guangxi Autonomous Region 545006, People’s Republic of China

^3. Department of Reproductive Medicine Center, Liuzhou Maternity and Child Health Care Hospital, Liuzhou, Guangxi Autonomous Region, 545001, People’s Republic of China

^4. Department of Reproductive Medicine Center, The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530022, People’s Republic of China

14393381@qq.com

^* These authors contributed equally to this work.

Abstract: Cumulus cells (CCs) play an important role in oocyte mutation and development potential. They have been reported beneficial for oocyte selection, which is crucial for improving the success rate of assisted reproduction technology (ART). Here, we studied gene expression profile shifting in CCs affected by female ageing. CCs from 151 cases of intracytoplasmic sperm injection (ICSI) patients were collected, divided into younger (55 cases, ≤35 years old) and older (37 cases, 36-40 years old; 59 cases, >40 years old) groups. Based on ICSI results, the fertilization rate, embryo development rate and clinical pregnancy rate were negatively correlated with age. Using RNA sequencing, gene expression profile shifting occurred in CCs from different maternal ages. In total, 193 genes were differentially expressed between age groups. Gene ontology annotation shows that 64 genes were annotated to the developmental process and 20 genes were related to the reproductive process. We analysed differentially expressed genes related to reproduction and constructed protein interaction networks to selected crucial gene nodes. Furthermore, qRT-PCR validation was performed for four key node genes. This study provides a set of genes that might be beneficial for oocyte selection during in-vitro fertilization (IVF) and clinical outcomes.

[Wei L, Yang L, Zhang J, Zhou W, Li N. Intracytoplasmic sperm injection outcomes and gene profile alteration in human cumulus cells related to advanced maternal age. Nat Sci 2025,23(1):1-12]. ISSN 1545-0740 (print); ISSN 2375-7167 (online). http://www.sciencepub.net/nature 01. doi:10.7537/marsnsj230125.01

Keywords: Cumulus cells; Advanced maternal age; Assists reproduction technology; Transcriptome; Clinical outcomes

Full Text

1

2

Distribution Of Atmospheric Precipitation In The Middle Part Of The Zeravshan River Basin

Saidova D.A. ¹, Halimova G.S. ², Hikmatov F³.

¹Senior Lecturer, Department of Hydrometeorology and Environmental Monitoring,

Faculty of Hydrometeorology, Mirzo Ulugbek National University of Uzbekistan, ²Scientific supervisor, Doctor of Philosophy in Geographical Sciences (PhD),

Faculty of Natural Sciences, Department of Ecology and Geography,

³Professor of the Department of Terrestrial Hydrology at the Faculty of Hydrometeorology of the National University of Uzbekistan

E-mail: dono.saidova.76@gmail.com

Abstract. The article is devoted to the study of peculiarities of atmospheric precipitation distribution in the middle part of the Zeravshan river basin. For this purpose the reference meteorological observation points located on the territory of the studied basin are selected. A hyetographic curve characterising changes in the amount of precipitation with the altitude of the terrain is constructed.

[Saidova D.A., Halimova G.S., Hikmatov F. Distribution Of Atmospheric Precipitation In The Middle Part Of The Zeravshan River Basin. Nat Sci 2025,23(1):13-16]. ISSN 1545-0740 (print); ISSN 2375-7167 (online). http://www.sciencepub.net/nature 01. doi:10.7537/marsnsj230125.02

Key words: river; Zeravshan river basin; meteorological observation points; precipitation; hyetographic curve

Full Text

2

3

Delineation of Leachate at Oko-Filling Dumpsite Igando, Lagos, Nigeria Using Electrical Resistivity Methods

Ogungbe, A. S*, Hotonu E.O, Onori, E.O.

Department of Physics, Lagos State University, Lagos, Nigeria

abiola.ogungbe@lasu.rdu.ng, ogungbea@yahoo.co.uk

Abstract: Detailed knowledge of delineation of leachate at Oko-Filling dumpsite is crucial for improved groundwater quality management that serves the adjoining communities. The electrical resistivity methods were applied to investigate the extent at which contaminants from the dumpsite have infiltrated the subsurface lithology and the groundwater in the neighboring communities. Oko-Filling is situated between latitudes 6° 33’ 43’’ N and 6° 33’ 58’’ N and longitudes 3° 15’ E and 3° 15’ 20’’ E, in Alimosho Local Government Area of Lagos State, Southwest, Nigeria. Fifteen (15) vertical electrical soundings (VES) were carried out using Schlumberger array, while 2D electrical resistivity survey using Wenner array was conducted along five profiles for the survey. The VES data were interpreted using partial curve matching and computer iteration techniques and the pseudo section contouring method was used to plot the data from the 2D resistivity survey. The results of the VES showed that the subsurface of the area can be characterized into five different layers: topsoil, clay, sandy clay, clayey sand and sand. Resistivity of the study area varied between 3.7 and 1874 Ωm. The subsurface is also characterized by occurrence of near horizontal layering that typifies a sedimentary environment. The very low resistivity (<25 Ωm) portion of the clay layer in profile 2 may be due to leachate. The basal part of the clay unit in profile 3 is defined by an anomalously low resistivity zone (<30 Ωm), suggestive of contaminated zone.  The obvious anomalously low resistivity units that occupy most part of the 2D sections of profiles 4 and 5, is a clear indication of a contaminated zone. The water bearing units of the subsurface serving the nearby communities is contaminated to depths of about 42 m, which could pose serious health challenges to neighboring communities.

[Ogungbe, A. S, Hotonu E.O, Onori, E.O. Delineation of Leachate at Oko-Filling Dumpsite Igando, Lagos, Nigeria Using Electrical Resistivity Methods. Nat Sci 2025,23(1):17-25]. ISSN 1545-0740 (print); ISSN 2375-7167 (online). http://www.sciencepub.net/nature 03. doi:10.7537/marsnsj230125.03

Keywords: Delineation; Leachate; Oko-Filling; Dumpsite; Igando; Lagos; Nigeria; Electrical Resistivity Method

Full Text

3

4

Formulation and Characterization of Nanoemulsion Biopesticide from Balanite aegyptiaca Seed Kernel Extract

Aishatu Haruna^1*, Mahmud Yahya Jada¹, Gambo Abdullahi¹ and Ishaku Adamu Gali².

¹Department of Crop Protection, Faculty of Agriculture, Modibbo Adama University, (MAU) Yola, Adamawa State, Nigeria.

²Department of Biotechnology, Faculty of Life Sciences, Modibbo Adama University, (MAU) Yola, Adamawa State, Nigeria.

*For correspondence: aishaharun@mau.edu.ng

Abstract: Consumers' growing awareness and desire for food free of pesticide residues is what led to the incubation of this research work. The study aimed at developing a nanoemulsion formulation of Balanite aegyptiaca seed kernel extract for use as a biopesticide.  A preliminary study was carried out on the solubility and miscibility of surfactant, carrier, and deionized water utilizing the low-energy spontaneous emulsification method.  Information from the study was then used to plot a ternary phase diagram (TPD) showing both the isotropic zone and the anisotropic zones, from which a point with a surfactant, oil, and water ratio (20:20:60) was selected from the isotropic zone for the formulation.  The developed nanoemulsion was tested for stability and thermostability, and both results revealed the system was stable. Similarly, the physiochemical properties of the developed nanoemulsion system were characterized, and the results showed that the system showed the following properties: mean particle size of 173.2 ± 21.6, polydispersity index (PDI) of 0.403 ± 0.038, surface tension of 32.53 ± 0.71, viscosity of 37.33 ± 0.67 mPas, and a pH of 4.92 ± 0.02. The formulation appears homogeneous, clear, and transparent, which further indicates it’s a nanoemulsion formulation. Therefore, the nanoemulsion formulation of B. aegyptiaca seed kernel can serve as a safe and environmentally friendly substitute for synthetic pesticides in the management of plant diseases.

[Aishatu Haruna, Mahmud Yahya Jada, Gambo Abdullahi, Ishaku Adamu Gali. Formulation and Characterization of Nanoemulsion Biopesticide from Balanite aegyptiaca Seed Kernel Extract. Nat Sci 2025,23(1):26-34]. ISSN 1545-0740 (print); ISSN 2375-7167 (online). http://www.sciencepub.net/nature 04. doi:10.7537/marsnsj230125.04

Keywords: Nanoemulsion; Formulation; B. aegyptiaca; Seed kernel; Characterization; Extract

Full Text

4